Eae43^SJL/J
QTL Variant Detail

Summary

• QTL variant: Eae43^SJL/J
• Name: experimental allergic encephalomyelitis susceptibility 43; SJL/J
• MGI ID: MGI:2156763
• QTL: Eae43 Location: Chr7:68124954-68125093 bp Genetic Position: Chr7, Syntenic

Variant origin

• Strain of Specimen: SJL/J

Variant description

• Allele Type: QTL
• Inheritance: Dominant

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:49054

Authors isolated quantitative trait loci believed to be invloved with the disease frequency of experimental allergic encephalomyelitis (EAE). (SJL/J x B10.S/DvTe)F2; SJL/J (susceptible) and B10.S/DvTe (resistant) mice; were bred to create F2 progeny used in the analysis.

A genome wide scan of 291 affected and 390 unaffected animals indicated the presence of a locus mapping to mouse Chromosome 7 between D7Mit185 and D7Mit39, named Eae4 by the authors.

6.30.2015 Curator Note: Because Eae4 was originally mapped in J:29171 in 1995 using (ABH x NOD)F1 x NOD backcross mice; which differs from the cross used here; we consider the current study a separate mapping experiment and have named this QTL Eae43.

J:105972

QTL associated with the modification of experimental allergic encephalomyelitis (EAE) due to non-genetic factors, such as age and time of season, were mapped in 760 (B10.S/DvTe x SJL/J)F2 intercross animals using 170 microsatellite markers (average spacing = 10 cM) in the genome scan. Animals were immunized with mouse spinal cord homogenate at an average age of 15.3 +/- 10.5 weeks of age. Animals were monitored for symptoms of EAE from 10 to 60 days post-immunization.

Linkage to EAE susceptibility in 6- to 12-week old animals and summer cohorts coincided with previously identified QTL Eae43 at 50 cM (62.26 Mb; D7Mit233) on mouse Chromosome 7 and Eae5 at 21.1 cM (38.72 Mb; D17Mit105) on mouse Chromosome 17. Eae11 at 41 cM (69.73 Mb; D16Mit140) on mouse Chromosome 16 was also detected in this cohort.

When a summer cohort of 366 F2 animals at an average age of 31 +/- 12 weeks was analyzed, significant linkage to EAE susceptibility was detected at a novel locus on mouse Chromosome 4 near D4Mit221 (58 cM),D4Mit203 (60 cM), and D4Mit204 (61.9 cM). This locus is named Eae40 (author's designation is Eae36). Eae40 maps near several previously identified autoimmune loci, such as Lbw2 (42.6 cM), Lbm1 (26.3 cM), Arvm2 (58 cM), Sles2 (58 cM), Idd9.1 (60 cM), and Idd11 (64.6 cM). Potential candidate genes identified near the Eae40 interval are Csf3r (57.5 cM), Pnrc2 (66.7 cM), and Htr1d (66 cM). Amino acid substitutions are present in Pnrc2 (L41H) and Htr1d (A22V) protein sequences between inbred strains SJL/J andC57BL/10.

References

• Original: J:53193 Butterfield RJ, et al., Genetic analysis of disease subtypes and sexual dimorphisms in mouse experimental allergic encephalomyelitis (EAE): relapsing/remitting and monophasic remitting/nonrelapsing EAE are immunogenetically distinct. J Immunol. 1999 Mar 1;162(5):3096-102
• All: 3 reference(s)