Zic2^tm1Jaru
Targeted Allele Detail

Summary

• Symbol: Zic2^tm1Jaru
• Name: zinc finger protein of the cerebellum 2; targeted mutation 1, Jun Aruga
• MGI ID: MGI:2156825
• Synonyms: Zic2^kd
• Gene: Zic2 Location: Chr14:122712847-122717264 bp, + strand Genetic Position: Chr14, 65.97 cM
• Alliance: Zic2^tm1Jaru page

Neural tube defects found in Zic2^tm1Jaru/Zic2^tm1Jaru mice

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60644
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutation: Insertion
• Mutation details: A gene targeting event was designed to replace the first exon containing the start codon and encoding three of the five zinc fingers. However, unexpected recombination resulted in an allele with reduced expression. The allele retained exon 1 with the neomycin-resistance marker in the first intron, shown by Southern blot. This resulted in reduction (knockdown, kd) of Zic2 expression. (J:60644)

Disease models

Expression

In Mice Carrying this Mutation:	96 assay results
In Structures Affected by this Mutation:	25 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Zic2 Mutation: 41 strains or lines available

References

• Original: J:60644 Nagai T, et al., Zic2 regulates the kinetics of neurulation. Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1618-23
• All: 17 reference(s)