Ppp3r1^tm1Stl
Targeted Allele Detail

Summary

• Symbol: Ppp3r1^tm1Stl
• Name: protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type I); targeted mutation 1, Susumu Tonegawa
• MGI ID: MGI:2156983
• Synonyms: fCNB1, PP2B-loxP
• Gene: Ppp3r1 Location: Chr11:17109298-17150380 bp, + strand Genetic Position: Chr11, 9.71 cM, cytoband A3.1
• Alliance: Ppp3r1^tm1Stl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73203
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: Homologous recombination flanked the three major coding exons, exons 2-4, with a loxP site and a LFNT cassette. The LFNT cassette consisted of PGK-neomycin and HSV-thymidine kinase selection sequences flanked by a pair of loxP sites and a pair of frt sites. Cre- or Flp-mediated recombination in ES cells removed the PGK-neomycin and HSV-thymidine kinase sequences prior to production of chimeras. (J:73203)

Disease models

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ppp3r1 Mutation: 30 strains or lines available

References

• Original: J:73203 Zeng H, et al., Forebrain-Specific Calcineurin Knockout Selectively Impairs Bidirectional Synaptic Plasticity and Working/Episodic-like Memory. Cell. 2001 Nov 30;107(5):617-29
• All: 27 reference(s)