Otx2^tm3(Emx2)Sia
Targeted Allele Detail

Summary

• Symbol: Otx2^tm3(Emx2)Sia
• Name: orthodenticle homeobox 2; targeted mutation 3, Shinichi Aizawa
• MGI ID: MGI:2157061
• Synonyms: Otx2^Emx2
• Gene: Otx2 Location: Chr14:48894238-48905101 bp, - strand Genetic Position: Chr14, 25.36 cM
• Alliance: Otx2^tm3(Emx2)Sia page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70745
• Parent Cell Line: TT2 (ES Cell)
• Strain of Origin: (C57BL/6NCrlj x CBA/JNCrlj)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: An Emx2 cDNA was inserted into the Otx2 gene as follows: the Emx2 cDNA was inserted at the start codon of the Otx2 gene followed by a loxP-flanked neomycin selection cassette. Resulting chimeric mice were bred to females expressing Cre in resulting zygotes, and the F1 generation was screened for the removal of the neomycin selection cassette. (J:70745)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Otx2 Mutation: 50 strains or lines available

References

• Original: J:70745 Suda Y, et al., Emx2 directs the development of diencephalon in cooperation with Otx2. Development. 2001 Jul;128(13):2433-50
• All: 1 reference(s)