Trp53^tm1Holl
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Holl
• Name: transformation related protein 53; targeted mutation 1, Monica Hollstein
• MGI ID: MGI:2157167
• Synonyms: HUPKI, Hupki R72, p53^72arg, p53^hki, p53^KI
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Holl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68918
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Insertion of a loxP-flanked neomycin-thymidine kinase expression cassette replaced the mouse DNA-binding domain (exons 4-9) with the corresponding homologous segment of the normal human gene, leaving transcription under control of the endogenous promoter. Cre-mediated recombination in ES cells excised the neo-tk sequences. cDNA sequencing studies determined that transcripts from spleen of homozygous mutant mice carried the human sequence for exons 4-9, and that the gene is correctly spliced and transcribed. (J:68918)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:68918 Luo JL, et al., Knock-in mice with a chimeric human/murine p53 gene develop normally and show wild-type p53 responses to DNA damaging agents: a new biomedical research tool. Oncogene. 2001 Jan 18;20(3):320-8
• All: 18 reference(s)