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Trp53tm1Holl
Targeted Allele Detail
Summary
Symbol: Trp53tm1Holl
Name: transformation related protein 53; targeted mutation 1, Monica Hollstein
MGI ID: MGI:2157167
Synonyms: HUPKI, Hupki R72, p5372arg, p53hki, p53KI
Gene: Trp53  Location: Chr11:69471185-69482699 bp, + strand  Genetic Position: Chr11, 42.83 cM, cytoband B2-C
Alliance: Trp53tm1Holl page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:68918
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Humanized sequence)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsInsertion of a loxP-flanked neomycin-thymidine kinase expression cassette replaced the mouse DNA-binding domain (exons 4-9) with the corresponding homologous segment of the normal human gene, leaving transcription under control of the endogenous promoter. Cre-mediated recombination in ES cells excised the neo-tk sequences. cDNA sequencing studies determined that transcripts from spleen of homozygous mutant mice carried the human sequence for exons 4-9, and that the gene is correctly spliced and transcribed. (J:68918)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 2 anatomical structure(s)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 0 lines available
Carrying any Trp53 Mutation:  239 strains or lines available
References
Original:  J:68918 Luo JL, et al., Knock-in mice with a chimeric human/murine p53 gene develop normally and show wild-type p53 responses to DNA damaging agents: a new biomedical research tool. Oncogene. 2001 Jan 18;20(3):320-8
All:  18 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory