Apoe^{tm2(APOE*3)Mae}
Targeted Allele Detail

Summary

• Symbol: Apoe^{tm2(APOE*3)Mae}
• Name: apolipoprotein E; targeted mutation 2, Nobuyo Maeda
• MGI ID: MGI:2157240
• Synonyms: 3, Apoe³, APOE*3, APOE3, apoE3-KI, apoE3 TR, Apoe^tm2(APOE)Mae, apoE TR, E3, TgH^APOE3N8, TgH/ApoeE3/N8, TRE3
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^{tm2(APOE*3)Mae} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:41705
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Modified isoform(s), Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Apoe^{tm2(APOE*3)Mae} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

  Expresses the epsilon3 isoform

• Mutation details: A DNA fragment containing exons 2-4 of the human APOE gene (the APOE3 isoform) replaced an equivalent portion of the mouse Apoe gene. Western blot analysis on plasma derived from homozygous mice demonstrated that the human protein was expressed from this allele and the endogenous mouse protein was not detectable. (J:41705)

Expression

In Mice Carrying this Mutation:

3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 156 strains or lines available

References

• Original: J:41705 Sullivan PM, et al., Targeted replacement of the mouse apolipoprotein E gene with the common human APOE3 allele enhances diet-induced hypercholesterolemia and atherosclerosis. J Biol Chem. 1997 Jul 18;272(29):17972-80
• All: 176 reference(s)