Dob2^AKR/J
QTL Variant Detail

Summary

• QTL variant: Dob2^AKR/J
• Name: dietary obesity 2; AKR/J
• MGI ID: MGI:2157551
• QTL: Dob2 Location: Chr9:86121898-120118690 bp Genetic Position: Chr9, cM position of peak correlated region/allele: 59.33 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: AKR/J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers decreased adiposity compared to SWR/J.
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Candidate Genes

J:91835

The correlation between gene expression and dietary interaction was examined in a population of (BALB/cStCrlfC3H/Nctr x VY/WffC3Hf/Nctr-A(vy)/a)F1 animals. A/a and A/A^vy animals were placed on a 70% (calorie restricted) or 100% (non-restricted) diet andliver mRNA levels were assessed for over 18,000 genes using DNA microarrays. Twenty-eight known genes showing statistically significant differential expression between the 70% and 100% calorie diets and A/a and A/A^vy genotypes mapped near known diabesity QTLs. These genes may be considered further for candidate genes.

On mouse Chromosome 9, Ets1 (15 cM) showed differential expression between the restricted and unrestricted diets on the A/A^vy background and between A/a and A/A^vy animals on both therestricted and unrestricted diets. Diabesity QTLs mapping near Ets1 are Bwtq4 (8 cM) and Obq5 (19 cM). A candidate gene for diabesity QTL Dob2 (60 cM) is Cacna2d2 (60 cM). Cacna2d2 shows differential expression between the restricted and unrestricted diets on a A/a background.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:20700

Analysis of 189 (AKR x SWR/J) F2 mice indicates that a QTL (Dob2) affecting dietary obesity in AKR/J mice is located on Chromosome 9 between D9Mit11 and D9Mit18 with a peak LOD score of 4.85. The 189 animals used for statistical analysis represent the 10% tails of the phenotype distribution for the total F2 population (931 animals) and may not accurately represent the percentage of variance controlled by this locus. Backcross analysis did not confirm the association of Dob2 with loci on Chromosome 9.

References

• Original: J:20700 West DB, et al., Dietary obesity linked to genetic loci on chromosomes 9 and 15 in a polygenic mouse model. J Clin Invest. 1994 Oct;94(4):1410-6
• All: 1 reference(s)