Il1b^tm1Dch
Targeted Allele Detail

Summary

• Symbol: Il1b^tm1Dch
• Name: interleukin 1 beta; targeted mutation 1, David D Chaplin
• MGI ID: MGI:2157804
• Synonyms: IL-1beta-, IL-1BtmChaplin
• Gene: Il1b Location: Chr2:129206490-129213059 bp, - strand Genetic Position: Chr2, 62.97 cM, cytoband F
• Alliance: Il1b^tm1Dch page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:33465
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A PGK-neomycin resistance cassette was inserted into exon 4 that resulted in an in-frame termination codon upstream of the ICE cleavage site. The authors predict that the receptor-binding domain of the protein would not be translated. Northern blot analysis detected no transcripts in LPS-stimulated peritoneal exudate cells from homozygous mice. Pulse-chase labeling and immunoprecipitation studies of thioglycollate-elicited PEC demonstrated that no detectable protein was present in homozygous mice. ELIZA analysis of ATP-treated PEC also did not detect protein produced by this allele. (J:33465)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Il1b Mutation: 25 strains or lines available

References

• Original: J:33465 Shornick LP, et al., Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone. J Exp Med. 1996 Apr 1;183(4):1427-36
• All: 50 reference(s)