Szs13^DBA/2J
QTL Variant Detail

Summary

• QTL variant: Szs13^DBA/2J
• Name: seizure susceptibility 13; DBA/2J
• MGI ID: MGI:2157850
• QTL: Szs13 Location: Chr15:68685832-68694449 bp Genetic Position: Chr15, Syntenic

Variant origin

• Strain of Specimen: DBA/2J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased seizure susceptibility compared to C57BL/6J. (J:70295)
• Inheritance: Dominant

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:70295

A (C57BL/6J x DBA/2J)F2 intercross was used to map QTLs involved in seizure susceptibility in response to electroshock stimulus. DBA/2J exhibits a lower maximal electroshock seizure threshold (MEST) than C57BL/6J. F1 and F2 animals exhibit intermediate MEST values. Seizure susceptibility also appears to be a sex-influenced trait. C57BL/6J and F2 female animals have significantly lower MEST values than males. A major QTL was mapped to Chromosome 1 (79 cM) with a LOD score of 12.6 near D1Mit150. This QTL mapped to the same position as Szs1, a QTL for PZT-induced seizure susceptibility. Congenic strain B6.D2-Mtv7a/Ty(Mtv7) verified the Szs1 locus on Chromosome 1. B6.D2-Mtv7a/Ty(Mtv7) carries a small portion of DBA/2J-like DNA on Chromosome 1, including D1Mit150, on a C57BL/6J background. The congenic exhibits similar MEST values and susceptibility to PZT-induced seizures as DBA/2J. Other major loci mapped to Chromosome 2 (Szs7) with a LOD of 3.7 spanning 1 cM - 15.3 cM, Chromosome 5 (Szs11) with a LOD of 6.8 spanning 8 cM - 39 cM, and Chromosome 15 (Szv3, a previously mapped QTL for kainic acid-induced seizure susceptibility) with a LOD of 5.0 spanning 6.7 cM - 22.2 cM. Szs13 also mapped to Chromosome 15 with LOD = 5.0 spanning 46.9 cM - 52.2 cM. Suggestive loci were also identified: Szs8 (LOD = 2.9) on chromosome 2 spanning 86 cM - 107cM, Szs9 (LOD = 2.9) on Chromosome 10 spanning 27 cM - 40 cM, Szs10 (LOD = 3.2) on Chromosome 11 spanning 20 cM - 34.25 cM, and Szs12 (LOD = 2.9) on Chromosome 13 spanning 45cM - 59 cM.

J:166489

The genetic influences on seizure susceptibility in the C57BL/6J (susceptible) and DBA/2J inbred mouse strains were studied in various congenic strains constructed between the inbreds. The mapping of Szs13 to mouse chromosome 15 was confirmed to an approximate 9 Kb region between rs31267792 (Chr15:68644413 (NCBI Build 37)) and rs31271969(Chr15:68653030 (NCBI Build 37)).

References

• Original: J:70295 Ferraro TN, et al., Quantitative genetic study of maximal electroshock seizure threshold in mice: evidence for a major seizure susceptibility locus on distal chromosome 1. Genomics. 2001 Jul;75(1-3):35-42
• All: 1 reference(s)