Wta4^LG/J
QTL Variant Detail

Summary

• QTL variant: Wta4^LG/J
• Name: weight adult 4; LG/J
• MGI ID: MGI:2157919
• QTL: Wta4 Location: Chr17:33819721-33819843 bp Genetic Position: Chr17, cM position of peak correlated region/allele: 17.98 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: LG/J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased body weight compared to SM/J. (J:62610)
• Inheritance: Other (see notes)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Wta4 participates in additive interactions with other body weight QTLs (Wta1, Wta2, Wta3) and appears to exhibit pleiotropic effects.

Candidate Genes

J:99477

Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes.

Potential candidate genes for Hdl4 (32.3 cM) on mouse Chromosome 17 as identified by ExQuest are Gnmt, Lrg1, and Sepx1 (10 cM). For QTL Insq5 (56.7 cM), potential candidate gene Abcg5 (54.5 cM) was identified. For QTL Obq4 (4 cM) and Wta4 (17 cM), potential candidate genes Plg (7.3 cM), Acat2 (7.55 cM), Acat3 (7.55 cM), Hagh (11 cM), Igfals, Decr2, Clps (17.1 cM), Tff1 (17 cM), Tff2 (17 cM), Tff3 (17 cM), and Apom were identified. Tff3 exhibits almost undetectable levels of transcription in the liver of Tally Ho animals compared C57BL/6J.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:62610

96 microsatellite markers were screened in 510 animals from a LG/J by SM/J F2 intercross to identify loci associated with adiposity, body weight, and tail length phenotypes. The LG/J inbred strain has a large body size and the SM/J inbred strain has a small body size. Linkage for adiposity was detected on mouse chromosome 1 (Adip1, LPR = 2.99 at D1Mit3) from 4 cM - 16 cM, Chromosome 6 (Adip2, LPR = 6.23 at D6Nds5) from 74 cM - 88 cM, Chromosome 7 (Adip3, LPR = 9.88 at D7Mit148) from 48 cM - 54 cM, Chromosome 8 (Adip4, LPR = 3.8 at D8Mit25) from 14 cM-40 cM, Chromosome 9 (Adip5, LPR = 1.84 at D9Mit8) from 38 cM - 76 cM, Chromosome 12 (Adip6, LPR = 3.78 at D12Mit6) from 48 cM - 58 cM, Chromosome 13 (Adip7, LPR = 1.9 at D13Mit1) from 0 cM - 30 cM, and Chromosome 18 (Adip8, LPR = 2.84 at D18Mit17) from 8 cM - 38 cM. LG/J-derived alleles contribute to higher adiposity except at Adip5 and Adip6 where LG/J-derived alleles contribute to lower adiposity. All adiposity QTLs are involved in epistatic interactions, and some adiposity QTLs show sex influence. Linkage for adult body weight was detected on mouse chromosome 4 (Wta1, LPR = 2.35 at D4Mit2) from 0 cM - 30 cM, Chromosome 6 (Wta2, LPR = 3.49 at D6Mit58) from 90 cM - 96 cM, Chromosome 14 (Wta3, LPR = 2.70 at D14Nds1) from 0 cM - 30 cM, and Chromosome 17 (Wta4, LPR = 2.44 at D17Mit16) from 14 cM - 42 cM. LG/J-derived alleles contribute to higher body weight at all body weight QTLs. Wta1-4 appear to interact additively and have pleiotropic effects. Some body weight QTLs show sex-specificity. Linkage for tail length (skeletal length) was detected on mouse Chromosome 1 (Skl1, LPR = 3.46 at D1Mit7) from 42 cM - 68 cM, Chromosome 2 (Skl2, LPR = 5.93 at D2Mit17) from 80 cM - 104 cM, chromosome 6 (Skl3, LPR = 8.52 atD6Mit9) from 36 cM - 58 cM, Chromosome 10 (Skl4, LPR = 4.93 at D10Mit133) from 68 cM - 84 cM, Chromosome 11 (Skl5, LPR = 3.32 at D11Mit15) from 72 cM - 92 cM, Chromosome 13 (Skl6, LPR = 5.06 at D13Mit9) from 74 cM - 104 cM, and Chromosome 16 (Skl7, LPR =3.7 at D16Mit2) from 0 cM - 24 cM. LG/J-derived alleles contribute to longer tails except at Adip6 and Skl7, and all tail length QTLs are involved in epistatic interactions.

References

• Original: J:62610 Cheverud JM, et al., Genetic architecture of adiposity in the cross of LG/J and SM/J inbred mice. Mamm Genome. 2001 Jan;12(1):3-12
• All: 1 reference(s)