Insq9^C57BL/6
QTL Variant Detail

Summary

• QTL variant: Insq9^C57BL/6
• Name: insulin QTL 9; C57BL/6
• MGI ID: MGI:2157922
• QTL: Insq9 Location: Chr10:82490938-82491121 bp Genetic Position: Chr10, cM position of peak correlated region/allele: 39.72 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/6

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers susceptibility to hyperinsulinemia compared to 129/SvIr. (J:61489)
• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Notes

Candidate Genes

J:99477

Authors used novel data mining tool ExQuest to identify novel candidate genes for existing diabesity QTLs. Next, candidate gene expression in the liver, adipose, and pancreas of diabesity-prone Tally Ho mice and diabesity-resistant C57BL/6J mice was assessed by quantitative PCR analysis. Several potential candidate genes, some with no previous association to diabesity QTLs, were identified. Since QTL intervals may be large and could contain hundreds or thousands of potential candidate genes, this method allows researchers to focus on those with strong potential as well as identify novel candidate genes.

Potential candidate genes for Bgeq8 (57 cM) on mouse Chromosome 10 as identified by ExQuest are Rdh7 and Hsd17b9. Hsd17b9 exhibits 9.5-fold increased expression in the liver of Tally Ho compared to C57BL/6J animals. For QTLs Insq9 (44 cM) and Igfbp3q2 (46 cM), potential candidate genes Ass1 (20 cM Chr 2)), Ftcd, Col18a1 (41.3 cM), Itgb2 (41.5 cM), Agpat3 (41.8 cM), Ndufs7, Oaz1 (43 cM), Pah (47 cM), Igf1 (48 cM), Nr1h4 (50 cM), and Kitl (57 cM) were identified.

Mapping and Phenotype information for this QTL, its variants and associated markers

J:61489

A genome scan with an average marker resolution of 20 cM was conducted on 6 month old male mice from a (C57BL/6 x 129SvIR)F2 intercross to map QTLs affecting susceptibility to hyperinsulinemia. Female mice of either strain do not develop hyperinsulinemia and were excluded from the study. The 129SvIR strain carries a mutation in the insulin receptor gene on Chromosome 8 and develops extreme hyperinsulinemia (elevated blood insulin). C57BL/6 does not develop hyperinsulinemia with or without mutation to the insulin receptor gene. The authors mapped 2 major QTLs involved in modulating blood insulin levels: Insq7 on Chromosome 2 spanning 27.6 cM - 42.9 cM (LOD = 5.58 at D2Mit151) and Insq9 on Chromosome 10 spanning 29 cM - 69 cM (LOD = 5.58 at D10Mit42). Also mapped were 3 suggestive QTLs: Insq6 on Chromosome 1 spanning 38.3 cM - 59.7 cM (LOD = 3.58 at D1Mit19), Insq8 on Chromosome 6 at 56.6 cM (LOD = 2.79 at D6Mit201), and Insq10 on Chromosome 12 at 44.1 cM (LOD = 3.11 at D12Mit231). Insq7 on Chromosome 2 and Insq9 on Chromosome 10 span a broad region and may contain multiple genes modulating plasma insulin levels.

References

• Original: J:61489 Kido Y, et al., Genetic modifiers of the insulin resistance phenotype in mice. Diabetes. 2000 Apr;49(4):589-96
• All: 1 reference(s)