Cia8^DBA/1J
QTL Variant Detail

Summary

• QTL variant: Cia8^DBA/1J
• Name: collagen induced arthritis QTL 8; DBA/1J
• MGI ID: MGI:2157964
• QTL: Cia8 Location: Chr10:51493400-82491121 bp Genetic Position: Chr10, cM position of peak correlated region/allele: 38.48 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: DBA/1J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased arthritis severity compared to C57BL/10SnSg. (J:57012)
• Inheritance: Recessive

Notes

Candidate Genes

J:81372

Microarray gene expression analysis was used to identify candidate genes for QTLs associated with collagen-induced arthritis. RNA from inflamed paws of collagen-treated mice were used in the microarray analysis and compared to RNA from normal paws. 223 genes showed a four-fold or greater expression difference between inflamed and normal paws. Nine of these genes are located near previously identified arthritis severity/susceptibility QTLs and may be considered for candidates.

For Cia1 on mouse Chromosome 17 (18.6 cM near the H2 locus) differential expression was observed for H2-Eb1 (18.66 cM) and H2-T23 (19.73 cM).

For Cia2 and Cia4 on mouse Chromosome 2 (both are located at 34 cM) differential expression was observed for Fap (36 cM) and Gcg (36 cM).

For Cia3 (48.7 cM) and Cia6 (30 cM) on mouse Chromosome 6 differential expression was observed for Raf1 (52.5 cM) and Slc6a6 (38.2 cM), respectively.

For Cia5 (40 cM) on mouse Chromosome 4 differential expression was observed for Ctsk (47.9 cM) and Cd53(50.5 cM).

For Cia8 (40.4 cM) on mouse Chromosome 10 differential expression was observed for Bsg (42.4 cM).

Mapping and Phenotype information for this QTL, its variants and associated markers

J:57012

To identify collagen-induced arthritis susceptibility loci outside of the MHC, crosses between the DBA/1 and the less susceptible B10.Q strain were analyzed.

Analysis of 224 (B10.Q x DBA/1)F2 mice identified a suggestive, recessive locus on chromosome 10, Cia8, was associated with disease severity (LOD = 3.3) and incidence (p = 0.002). Figure 2 displays the QTL mapping between D10Mit42 and D10Mit141. The disease promoting allele inherited from the DBA/1 strain. Putative candidate genes include Col10a1,Col8a1, Col6a1 and Col6a2.

References

• Original: J:57012 Yang HT, et al., Identification of genes controlling collagen-induced arthritis in mice: striking homology with susceptibility loci previously identified in the rat. J Immunol. 1999 Sep 1;163(5):2916-21
• All: 1 reference(s)