Atp4a^tm1Ges
Targeted Allele Detail

Summary

• Symbol: Atp4a^tm1Ges
• Name: ATPase, H+/K+ exchanging, gastric, alpha polypeptide; targeted mutation 1, Gary E Shull
• MGI ID: MGI:2158246
• Synonyms: H/Kalpha^-
• Gene: Atp4a Location: Chr7:30411634-30424959 bp, + strand Genetic Position: Chr7, 19.2 cM
• Alliance: Atp4a^tm1Ges page

Disruption of the gastric mucosa architecture in Atp4a^tm1Ges/Atp4a^tm1Ges mice

Show the 5 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63470
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A part of exon 8 encoding amino acids 360-390 was replaced with a neomycin selection cassette. Northern blot analysis demonstrated that no detectable full length transcript was produced from this allele in stomach of homozygous mice, although trace levels of a shorter 1 kb transcript was detected. Immunocytochemistry experiments on stomach tissue of homozygous mice demonstrated that no detectable protein was present. (J:63470)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Atp4a Mutation: 63 strains or lines available

References

• Original: J:63470 Spicer Z, et al., Stomachs of mice lacking the gastric H,K-ATPase alpha -subunit have achlorhydria, abnormal parietal cells, and ciliated metaplasia. J Biol Chem. 2000 Jul 14;275(28):21555-65
• All: 21 reference(s)