S1pr1^tm1Rlp
Targeted Allele Detail

Summary

• Symbol: S1pr1^tm1Rlp
• Name: sphingosine-1-phosphate receptor 1; targeted mutation 1, Richard L Proia
• MGI ID: MGI:2158346
• Synonyms: Edg1^LacZ, S1P1^-, S1P1^Ko, S1pr1^-, Slp1^-LacZ
• Gene: S1pr1 Location: Chr3:115504082-115508704 bp, - strand Genetic Position: Chr3, 50.0 cM
• Alliance: S1pr1^tm1Rlp page

Bleeding phenotypes of mice carrying different combinations of deleted S1p receptors

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71961
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: An IRES-lacZ reporter gene and neomycin selection cassette was inserted after codon 42 of the coding region of the gene. RT-PCR analysis demonstrated that the endogenous transcript was not detectable in homozygous embryos. A beta-galactosidase protein was expressed from this allele under the control of the endogenous promoter. (J:71961)

Expression

In Mice Carrying this Mutation:	12 assay results
In Structures Affected by this Mutation:	15 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any S1pr1 Mutation: 32 strains or lines available

References

• Original: J:71961 Liu Y, et al., Edg-1, the G protein-coupled receptor for sphingosine-1-phosphate, is essential for vascular maturation. J Clin Invest. 2000 Oct;106(8):951-61
• All: 31 reference(s)