Kifc2^tm1Gsn
Targeted Allele Detail

Summary

• Symbol: Kifc2^tm1Gsn
• Name: kinesin family member C2; targeted mutation 1, Lawrence SB Goldstein
• MGI ID: MGI:2158351
• Gene: Kifc2 Location: Chr15:76544058-76552396 bp, + strand Genetic Position: Chr15, 36.23 cM, cytoband D-E
• Alliance: Kifc2^tm1Gsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68003
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 4.0 kb genomic fragment containing the coding sequences for amino acids 31 to 560 was replaced with a neomycin selection cassette. These sequences correspond to the tail and alpha coiled coil domains and half of the motor domain. Northern blot analysis on RNA derived from brain of homozygous mice confirmed that no detectable transcript was produced from this allele. In addition, western blot analysis demonstrated that no encoded protein was detectable in brain lysates derived from homozygous mice. (J:68003)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Kifc2 Mutation: 25 strains or lines available

References

• Original: J:68003 Yang Z, et al., Functional analysis of mouse c-terminal kinesin motor kifc2. Mol Cell Biol. 2001 Apr;21(7):2463-6
• All: 2 reference(s)