Prkar2a^tm1Gsm
Targeted Allele Detail

Summary

• Symbol: Prkar2a^tm1Gsm
• Name: protein kinase, cAMP dependent regulatory, type II alpha; targeted mutation 1, G Stanley McKnight
• MGI ID: MGI:2158358
• Synonyms: RIIalpha KO
• Gene: Prkar2a Location: Chr9:108569342-108627643 bp, + strand Genetic Position: Chr9, 59.63 cM
• Alliance: Prkar2a^tm1Gsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74250
• Parent Cell Line: REK3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing the promoter sequences and the sequences encoding the first 27 amino acids of the encoded protein was replaced with a neomycin resistance gene. Western blot analysis on extracts of brain, testis, heart and skeletal muscle derived from homozygous mice confirmed that no detectable protein was produced from this allele. (J:74250)

Tumor Data

List all tumor models in MMHCdb carrying Prkar2a^tm1Gsm

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Prkar2a Mutation: 73 strains or lines available

References

• Original: J:74250 Burton KA, et al., Type II regulatory subunits are not required for the anchoring-dependent modulation of Ca2+ channel activity by cAMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1997 Sep 30;94(20):11067-72
• All: 8 reference(s)