Slc12a1^tm1Tkh
Targeted Allele Detail

Summary

• Symbol: Slc12a1^tm1Tkh
• Name: solute carrier family 12, member 1; targeted mutation 1, Nobuyuki Takahashi
• MGI ID: MGI:2158359
• Synonyms: NKCC2-, Slc12a1^tm1Unc
• Gene: Slc12a1 Location: Chr2:124994430-125071922 bp, + strand Genetic Position: Chr2, 61.23 cM
• Alliance: Slc12a1^tm1Tkh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62224
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 12 kb genomic fragment containing the promoter and exons 1-3 was replaced with a neomycin selection cassette. Western blot analysis on kidney proteins derived from homozygous mice demonstrated that no protein is produced from this allele. (J:62224)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Slc12a1 Mutation: 55 strains or lines available

References

• Original: J:62224 Takahashi N, et al., Uncompensated polyuria in a mouse model of Bartter's syndrome. Proc Natl Acad Sci U S A. 2000 May 9;97(10):5434-9
• All: 4 reference(s)