Apoe^{tm3(APOE*4)Mae}
Targeted Allele Detail

Summary

• Symbol: Apoe^{tm3(APOE*4)Mae}
• Name: apolipoprotein E; targeted mutation 3, Nobuyo Maeda
• MGI ID: MGI:2158398
• Synonyms: 4, apoe4, Apoe⁴, APOE*4, apoE4-KI, apoE4 TR, Apoe^tm3(APOE)Mae, E4, TgH/ApoE4/N8, TRE4
• Gene: Apoe Location: Chr7:19430034-19433113 bp, - strand Genetic Position: Chr7, 9.94 cM
• Alliance: Apoe^{tm3(APOE*4)Mae} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74417
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence)
• Mutations: Insertion, Intragenic deletion
• Apoe^{tm3(APOE*4)Mae} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOE (348)

  Apoe	(MGI:88057)

• Mutation details: A DNA fragment containing exons 2-4 of the human APOE gene (the APOE4 isoform) replaced an equivalent portion of the mouse Apoe gene. Western blot analysis on plasma derived from homozygous mice demonstrated that the human protein was expressed from this allele and the endogenous mouse protein was not detectable. (J:74417)

Disease models

Expression

In Mice Carrying this Mutation:

3 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Apoe Mutation: 156 strains or lines available

References

• Original: J:74417 Knouff C, et al., Apo E structure determines VLDL clearance and atherosclerosis risk in mice. J Clin Invest. 1999 Jun;103(11):1579-86
• All: 182 reference(s)