Prf1^tm1Tsc
Targeted Allele Detail

Summary

• Symbol: Prf1^tm1Tsc
• Name: perforin 1 (pore forming protein); targeted mutation 1, Jurg Tschopp
• MGI ID: MGI:2158418
• Synonyms: Kagi, Prf1^-
• Gene: Prf1 Location: Chr10:61133612-61140459 bp, + strand Genetic Position: Chr10, 32.18 cM
• Alliance: Prf1^tm1Tsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:21731
• Parent Cell Line: D3 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 3. RT-PCR and Northern blot analysis on splenoctye RNA derived from homozygous mice demonstrated that no transcript was produced from this allele. (J:21731)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prf1 Mutation: 51 strains or lines available

References

• Original: J:21731 Lowin B, et al., A null mutation in the perforin gene impairs cytolytic T lymphocyte- and natural killer cell-mediated cytotoxicity. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11571-5
• All: 11 reference(s)