Ptgs2^tm1Unc
Targeted Allele Detail

Summary

• Symbol: Ptgs2^tm1Unc
• Name: prostaglandin-endoperoxide synthase 2; targeted mutation 1, University of North Carolina
• MGI ID: MGI:2158457
• Synonyms: Cox2^-, COX-2-, PGHS-2-, Ptgs2^tm1Smi
• Gene: Ptgs2 Location: Chr1:149975851-149983978 bp, + strand Genetic Position: Chr1, 63.84 cM, cytoband H1
• Alliance: Ptgs2^tm1Unc page

Hypertoprhy of glomeruli in Ptgs2^tm1Unc/Ptgs2^tm1Unc mice that is not seen in Ptgs2^tm1.1Wls/Ptgs2^tm1.1Wls mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:29510
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette was inserted into exon 8. A 104 bp DNA fragment containing sequences encoding essential residues tyrosine 371 and histidine 374 was also deleted. Northern blot analysis on mRNA derived from LPS-induced macrophages from homozygous mice demonstrated that an aberrant 6.0 kb transcript is produced from this allele. However, western blot analysis confirmed that no detectable protein is produced from this allele in LPS-induced macrophages derived from homozygous mice. (J:29510)

Expression

In Structures Affected by this Mutation:

29 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ptgs2^tm1Unc

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptgs2 Mutation: 73 strains or lines available

Notes

BK4 is a subline of E14TG2a.

References

• Original: J:29510 Morham SG, et al., Prostaglandin synthase 2 gene disruption causes severe renal pathology in the mouse. Cell. 1995 Nov 3;83(3):473-82
• All: 141 reference(s)