Igh-8^tm1Schr
Targeted Allele Detail

Summary

• Symbol: Igh-8^tm1Schr
• Name: immunoglobulin heavy chain 8 (heavy chain of IgG3); targeted mutation 1, John R Schreiber
• MGI ID: MGI:2158494
• Synonyms: IgG3-, Igh-8^tm1Sch
• Gene: Igh-8 Location: Chr12:113329416-113330847 bp Genetic Position: Chr12, Syntenic
• Alliance: Igh-8^tm1Schr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49847
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 54 bp DNA fragment of exon CH1 was replaced with a neomycin selection cassette. ELISA assays demonstrated that no soluble IgG3 protein was detectable in serum derived from homozygous mice. (J:49847)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Igh-8 Mutation: 5 strains or lines available

References

• Original: J:49847 Shapiro DA, et al., Gamma 3 gene-disrupted mice selectively deficient in the dominant IgG subclass made to bacterial polysaccharides undergo normal isotype switching after immunization with polysaccharide-protein conjugate vaccines. J Immunol. 1998 Oct 1;161(7):3393-9
• All: 3 reference(s)