Prkar1b^tm1Gsm
Targeted Allele Detail

Summary

• Symbol: Prkar1b^tm1Gsm
• Name: protein kinase, cAMP dependent regulatory, type I beta; targeted mutation 1, G Stanley McKnight
• MGI ID: MGI:2158640
• Synonyms: RIbeta -
• Gene: Prkar1b Location: Chr5:139003061-139135756 bp, - strand Genetic Position: Chr5, 77.74 cM
• Alliance: Prkar1b^tm1Gsm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:28735
• Parent Cell Line: REK2 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 3 in reverse trancriptional orientation to the gene. Northern blot analysis demonstrated that no detectable trancript was present in total brain RNA derived from homozygous mice. Western blot analysis on protein lysates from cerebellum, neocortex and hippocampus of homozygous mice confirmed that no detectable protein is produced from this allele. (J:74419)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkar1b Mutation: 18 strains or lines available

References

• Original: J:28735 Brandon EP, et al., Hippocampal long-term depression and depotentiation are defective in mice carrying a targeted disruption of the gene encoding the RI beta subunit of cAMP-dependent protein kinase. Proc Natl Acad Sci U S A. 1995 Sep 12;92(19):8851-5
• All: 6 reference(s)