Srf^tm1Nor
Targeted Allele Detail

Summary

• Symbol: Srf^tm1Nor
• Name: serum response factor; targeted mutation 1, Alfred Nordheim
• MGI ID: MGI:2158696
• Gene: Srf Location: Chr17:46859255-46867101 bp, - strand Genetic Position: Chr17, 22.9 cM
• Alliance: Srf^tm1Nor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50876
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An 880 bp genomic fragment containing the initiation codon and sequences encoding the dimerization and DNA binding domains was replaced with a neomycin selection cassette. RT-PCR analysis showed that no detectable transcript was produced from this allele in mutant embryos (data not shown). Activity assays in extracts derived from homozygous E8.5 embryos demonstrated that no functional protein was present, and immunohistochemistry experiments on sections of E8.5 homozygous embryos confirmed that no encoded protein was detectable. (J:50876)

Expression

In Mice Carrying this Mutation:	68 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Srf Mutation: 27 strains or lines available

References

• Original: J:50876 Arsenian S, et al., Serum response factor is essential for mesoderm formation during mouse embryogenesis. EMBO J. 1998 Nov 2;17(21):6289-99
• All: 4 reference(s)