Cfb^tm1Mbo
Targeted Allele Detail

Summary

• Symbol: Cfb^tm1Mbo
• Name: complement factor B; targeted mutation 1, Marina Botto
• MGI ID: MGI:2158706
• Synonyms: B/C2^-, Bf/C2^-, C2/FB-, C2/fB KO
• Gene: Cfb Location: Chr17:35075350-35081492 bp, - strand Genetic Position: Chr17, 18.41 cM
• Alliance: Cfb^tm1Mbo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:45297
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 6 kb genomic fragment containing part of exon 1 through exon 18 was replaced with a promoterless lacZ gene linked to a neomycin selection cassette. Northern blot analysis on liver RNA derived from homozygous mice demonstrated that no detectable transcript was produced from this allele. Activity assays on serum derived from homozygous mice confirmed that no functional protein was made. (J:45297)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cfb Mutation: 29 strains or lines available

References

• Original: J:45297 Taylor PR, et al., A targeted disruption of the murine complement factor B gene resulting in loss of expression of three genes in close proximity, factor B, C2, and D17H6S45. J Biol Chem. 1998 Jan 16;273(3):1699-704
• All: 22 reference(s)