C3ar1^tm1Raw
Targeted Allele Detail

Summary

• Symbol: C3ar1^tm1Raw
• Name: complement component 3a receptor 1; targeted mutation 1, Rick A Wetsel
• MGI ID: MGI:2158707
• Synonyms: C3aR -, C3ar1^tm1RWe
• Gene: C3ar1 Location: Chr6:122824099-122833116 bp, - strand Genetic Position: Chr6, 57.93 cM, cytoband F1
• Alliance: C3ar1^tm1Raw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:68807
• Parent Cell Line: RW-4 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: DNA sequences encoding the entire open reading frame of the gene were replaced with a neomycin selection cassette. RT-PCR analysis on RNA derived from brain, kidney, liver, lung, testis and induced peritoneal macrophages demonstrated that no detectable transcript is produced from this allele. Immunocytochemistry and flow cytometry experiments confirmed that no detectable protein is expressed from this allele in induced peritoneal macrophages derived from homozygous mice. (J:68807)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any C3ar1 Mutation: 36 strains or lines available

References

• Original: J:68807 Kildsgaard J, et al., Cutting edge: targeted disruption of the C3a receptor gene demonstrates a novel protective anti-inflammatory role for C3a in endotoxin-shock. J Immunol. 2000 Nov 15;165(10):5406-9
• All: 37 reference(s)