Chrm1^tm1Nmn
Targeted Allele Detail

Summary

• Symbol: Chrm1^tm1Nmn
• Name: cholinergic receptor, muscarinic 1, CNS; targeted mutation 1, Neil M Nathanson
• MGI ID: MGI:2158709
• Synonyms: M1-, m1 deficient, M1R^-
• Gene: Chrm1 Location: Chr19:8641369-8660970 bp, + strand Genetic Position: Chr19, 5.44 cM
• Alliance: Chrm1^tm1Nmn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73591
• Parent Cell Line: REK3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 0.3 kb genomic fragment containing 5' flanking sequence, the initiation codon and the first 165 nucleotides of coding sequence was replaced with a neomycin selection cassette. Immunohistochemistry experiments on sections of hippocampus derived from homozygous mice confirmed that no detectable protein is produced from this allele. (J:73591)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrm1 Mutation: 27 strains or lines available

References

• Original: J:73591 Hamilton SE, et al., Disruption of the m1 receptor gene ablates muscarinic receptor-dependent M current regulation and seizure activity in mice. Proc Natl Acad Sci U S A. 1997 Nov 25;94(24):13311-6
• All: 20 reference(s)