Ptprs^tm1Dro
Targeted Allele Detail

Summary

• Symbol: Ptprs^tm1Dro
• Name: protein tyrosine phosphatase receptor type S; targeted mutation 1, Daniela Rotin
• MGI ID: MGI:2158734
• Synonyms: Ptprs-, PTPsigma^-
• Gene: Ptprs Location: Chr17:56719426-56783480 bp, - strand Genetic Position: Chr17, 29.32 cM
• Alliance: Ptprs^tm1Dro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:53433
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: Insertion of an IRES-beta-galactosidase-neomycin cassette into an intron interrupted the coding sequence between nt 460-850 in the first 1-2 immunoglobulin repeats. Northern analysis using a rat cDNA probe encompassing most of the extracellular domain did not detect transcript in heads of one-day-old homozygous mutant mice. Immunoblot analysis of brain immunoprecipitates using an affinity purified antibody against the intracellular juxtamembrane region did not detect protein in homozygous mutant newborn mice. A beta-galactosidase fusion protein was expressed by this allele under the control of the endogenous promoter. (J:53433)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ptprs Mutation: 78 strains or lines available

Notes

ES cell line = CJR8.8; strain of origin = 129 (substrain not specified)

References

• Original: J:53433 Wallace MJ, et al., Neuronal defects and posterior pituitary hypoplasia in mice lacking the receptor tyrosine phosphatase PTPsigma. Nat Genet. 1999 Mar;21(3):334-8
• All: 7 reference(s)