Ikzf1^tm1Kge
Targeted Allele Detail

Summary

• Symbol: Ikzf1^tm1Kge
• Name: IKAROS family zinc finger 1; targeted mutation 1, Katia Georgopoulos
• MGI ID: MGI:2158738
• Synonyms: DN^-, Ik7DN
• Gene: Ikzf1 Location: Chr11:11634980-11722926 bp, + strand Genetic Position: Chr11, 7.02 cM
• Alliance: Ikzf1^tm1Kge page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:20671
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Dominant negative)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An 8.5 kb genomic fragment containing part of exon 3 and exon 4 was replaced with a neomycin cassette. The mutation deletes zinc fingers 1, 2, and 3, which mediate the high affinity binding domain of the protein. Mutant mRNA was detected by RT-PCR analysis. Low amounts of mutant protein were detected by western analysis. Gel retardation assays using nuclear extracts from spleen and thymus showed no binding activity in homozygous mutant animals. A subsequent study suggested this allele generates a dominant-negative protein. (J:20671, J:40023)

Expression

In Mice Carrying this Mutation:	2 assay results
In Structures Affected by this Mutation:	15 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Ikzf1^tm1Kge

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ikzf1 Mutation: 30 strains or lines available

References

• Original: J:20671 Georgopoulos K, et al., The Ikaros gene is required for the development of all lymphoid lineages. Cell. 1994 Oct 7;79(1):143-56
• All: 19 reference(s)