Chrna9^tm1Bedv
Targeted Allele Detail

Summary

• Symbol: Chrna9^tm1Bedv
• Name: cholinergic receptor, nicotinic, alpha polypeptide 9; targeted mutation 1, Belen Elgoyhen and Douglas E Vetter
• MGI ID: MGI:2158742
• Synonyms: alpha9^-, alpha9 knockout, Chrna9^tm1Jbdv
• Gene: Chrna9 Location: Chr5:66092264-66134669 bp, + strand Genetic Position: Chr5, 33.84 cM
• Alliance: Chrna9^tm1Bedv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:55406
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 4 and flanking intronic sequences were replaced with a neomycin selection cassette. The deleted sequences encode the ligand-binding site and the first two transmembrane domains. RT-PCR analysis on nasal epithelium derived from homozygous mice confirmed that no detectable transcript containg sequences from exon 4 is produced from this allele. (J:55406)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrna9 Mutation: 40 strains or lines available

References

• Original: J:55406 Vetter DE, et al., Role of alpha9 nicotinic ACh receptor subunits in the development and function of cochlear efferent innervation. Neuron. 1999 May;23(1):93-103
• All: 32 reference(s)