Casp3^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Casp3^tm1Flv
• Name: caspase 3; targeted mutation 1, Richard Flavell
• MGI ID: MGI:2158753
• Synonyms: Casp3^-, caspase-3-, CPP32-
• Gene: Casp3 Location: Chr8:47070326-47092724 bp, + strand Genetic Position: Chr8, 26.39 cM
• Alliance: Casp3^tm1Flv page

Skeletal defects in Casp3^tm1Flv/Casp3^tm1Flv and Casp3^tm1Flv/Casp3⁺ mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:36821
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing sequences encoding a conserved motif was replaced with a neomycin selection cassette. RT-PCR analysis on samples derived from brain and liver of homozygous mice demonstrated that no detectable transcript is produced from this allele. Western blot analysis on samples derived from thymocytes of homozygous mice confirmed that no detectable protein was produced. (J:36821)

Expression

In Mice Carrying this Mutation:	23 assay results
In Structures Affected by this Mutation:	26 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Casp3 Mutation: 33 strains or lines available

References

• Original: J:36821 Kuida K, et al., Decreased apoptosis in the brain and premature lethality in CPP32-deficient mice. Nature. 1996 Nov 28;384(6607):368-72
• All: 88 reference(s)