Nos3^tm1Gdk
Targeted Allele Detail

Summary

• Symbol: Nos3^tm1Gdk
• Name: nitric oxide synthase 3, endothelial cell; targeted mutation 1, Axel Godecke
• MGI ID: MGI:2159003
• Synonyms: eNOS-
• Gene: Nos3 Location: Chr5:24569808-24589472 bp, + strand Genetic Position: Chr5, 11.32 cM
• Alliance: Nos3^tm1Gdk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:46061
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A genomic fragment containing exons 24 and part of 25 was replaced with a neomycin selection cassette. The deleted sequences encode the NADPH binding sites. Western blot analysis on extracts of heart, lung, kidney and liver, and immunohistochemistry experiments on homozygous mice confirmed that no stable protein was expressed from this allele. (J:46061, J:62375)

Disease models

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nos3 Mutation: 58 strains or lines available

References

• Original: J:46061 Godecke A, et al., Coronary hemodynamics in endothelial NO synthase knockout mice. Circ Res. 1998 Feb 9;82(2):186-94
• All: 30 reference(s)