Gja1^tm1Dlg
Targeted Allele Detail

Summary

• Symbol: Gja1^tm1Dlg
• Name: gap junction protein, alpha 1; targeted mutation 1, Brian R Duling
• MGI ID: MGI:2159007
• Synonyms: Cx43^fl
• Gene: Gja1 Location: Chr10:56253297-56266519 bp, + strand Genetic Position: Chr10, 28.64 cM
• Alliance: Gja1^tm1Dlg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:71092
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: LoxP sites were inserted flanking exon 2. A neomycin selection cassette and thymidine kinase gene that were also inserted 3' to the loxP sites were removed by transient cre recombinase expression in ES cells prior to the production of chimeric mice. Western blot and immunohistochemistry analysis on homozygous mice confirmed that there were no detectable changes in the expression of the encoded protein. (J:71092)

Disease models

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	16 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gja1 Mutation: 60 strains or lines available

References

• Original: J:71092 Liao Y, et al., Endothelial cell-specific knockout of connexin 43 causes hypotension and bradycardia in mice. Proc Natl Acad Sci U S A. 2001 Aug 14;98(17):9989-94
• All: 58 reference(s)