Cd86^tm2Shr
Targeted Allele Detail

Summary

• Symbol: Cd86^tm2Shr
• Name: CD86 antigen; targeted mutation 2, Arlene H Sharpe
• MGI ID: MGI:2159105
• Synonyms: B7-1/2-, B7-1/B7-2-, B7.1/B7.2-, B7DKO, B7KO, Cd80/86^-
• Gene: Cd86 Location: Chr16:36424231-36486443 bp, - strand Genetic Position: Chr16, 25.72 cM, cytoband B5
• Alliance: Cd86^tm2Shr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39089
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: To create a knockout of both Cd80 and Cd86 in mice, an ES cell line in which the Cd80 gene was mutated was subsequently used in a second round of gene targeting. A hygromycin selection cassette replaced an exon encoding the IgV-like domain of the protein and flanking genomic sequences of the Cd86 gene. FACS analysis on splenocytes and LPS-stimulated B cells derived from homozygous mice confirmed that no functional protein was encoded by this allele. (J:39089)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Cd86 Mutation: 39 strains or lines available

References

• Original: J:39089 Borriello F, et al., B7-1 and B7-2 have overlapping, critical roles in immunoglobulin class switching and germinal center formation. Immunity. 1997 Mar;6(3):303-13
• All: 121 reference(s)