Wasl^tm1Cgn
Targeted Allele Detail

Summary

• Symbol: Wasl^tm1Cgn
• Name: WASP like actin nucleation promoting factor; targeted mutation 1, University of Cologne
• MGI ID: MGI:2159337
• Synonyms: N-WASP^flox, Wasl^tm1Wehl
• Gene: Wasl Location: Chr6:24613804-24665008 bp, - strand Genetic Position: Chr6, 11.28 cM, cytoband A3
• Alliance: Wasl^tm1Cgn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73609
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A floxed neomycin resistance cassette was inserted upstream of exon 6 and a third loxP site was inserted downstream of exon 9. Transient expression of Cre recombinase in ES cells excised the neo cassette. Western analysis using polyclonal antibodies against the amino terminus detected wild-type protein in hippocampi from homozygous mice. (J:73609)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wasl Mutation: 39 strains or lines available

References

• Original: J:73609 Lommel S, et al., Actin pedestal formation by enteropathogenic Escherichia coli and intracellular motility of Shigella flexneri are abolished in N-WASP-defective cells. EMBO Rep. 2001 Sep;2(9):850-7
• All: 7 reference(s)