Mb^tm1Wlm
Targeted Allele Detail

Summary

• Symbol: Mb^tm1Wlm
• Name: myoglobin; targeted mutation 1, R Saunders Williams
• MGI ID: MGI:2159349
• Synonyms: Mb^-, myoglobin -
• Gene: Mb Location: Chr15:76899687-76934868 bp, - strand Genetic Position: Chr15, 36.36 cM
• Alliance: Mb^tm1Wlm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:50584
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced a genomic fragment containing exon 2, which encodes the haem-binding domain. RT-PCR analysis on RNA derived from hearts of homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot and immunohistochemical analyses confirmed that no stable protein was made from this allele in cardiac tissue of homozygous mice. (J:50584)

Expression

In Mice Carrying this Mutation:	33 assay results
In Structures Affected by this Mutation:	6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mb Mutation: 20 strains or lines available

References

• Original: J:50584 Garry DJ, et al., Mice without myoglobin. Nature. 1998 Oct 29;395(6705):905-8
• All: 7 reference(s)