Gabbr1^tm1Pngl
Targeted Allele Detail

Summary

• Symbol: Gabbr1^tm1Pngl
• Name: gamma-aminobutyric acid type B receptor subunit 1; targeted mutation 1, Menelas N Pangelos
• MGI ID: MGI:2159354
• Synonyms: GABAB1^-, GABAB1-KO
• Gene: Gabbr1 Location: Chr17:37356888-37385197 bp, + strand Genetic Position: Chr17, 19.16 cM
• Alliance: Gabbr1^tm1Pngl page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:70254
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An IRES-lacZ gene followed by a neomycin selection gene and thymidine kinase cassette replaced a genomic fragment containing exons 2-6. RT-PCR analysis on total brain RNA derived from homozygous mice demonstrated that no detectable transcript is produced from this allele. Western blot analysis on lysates of brain tissue of homozygous mice confirmed that no endogenous protein is expressed from this allele. However, brain histology revealed that a beta-galactosidase protein is expressed from this allele under the control of the endogenous promoter. (J:70254)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gabbr1 Mutation: 73 strains or lines available

References

• Original: J:70254 Prosser HM, et al., Epileptogenesis and enhanced prepulse inhibition in GABA(B1)-deficient mice. Mol Cell Neurosci. 2001 Jun;17(6):1059-70
• All: 4 reference(s)