Bak1^tm1Thsn
Targeted Allele Detail

Summary

• Symbol: Bak1^tm1Thsn
• Name: BCL2-antagonist/killer 1; targeted mutation 1, Craig B Thompson
• MGI ID: MGI:2159364
• Synonyms: bak-
• Gene: Bak1 Location: Chr17:27238786-27247601 bp, - strand Genetic Position: Chr17, 13.65 cM, cytoband B
• Alliance: Bak1^tm1Thsn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66872
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing exons 2-6. These exons encode the Bcl-2 homology domains of the encoded protein. Western blot analysis on spleen and thymus lysates derived from homozygous mice confirmed that no detectable protein is expressed from this allele. (J:66872)

Expression

In Mice Carrying this Mutation:

28 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Bak1 Mutation: 25 strains or lines available

References

• Original: J:66872 Lindsten T, et al., The combined functions of proapoptotic Bcl-2 family members bak and bax are essential for normal development of multiple tissues. Mol Cell. 2000 Dec;6(6):1389-99
• All: 170 reference(s)