Alcw3^C57BL/6J
QTL Variant Detail

Summary

• QTL variant: Alcw3^C57BL/6J
• Name: alcohol withdrawal 3; C57BL/6J
• MGI ID: MGI:2159389
• QTL: Alcw3 Location: unknown Genetic Position: Chr11, cM position of peak correlated region/allele: 25.18 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: C57BL/6J

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers increased alcohol withdrawal severity compared to DBA/2J. (J:36168)
• Inheritance: Not Specified

Notes

Candidate Genes

J:98595

Microarray analysis was used to identify genes showing alcohol-induced differential expression between inbred strains C57BL/6J and DBA/2J. These genes were then correlated to alcohol-related QTLs to identify potential candidate genes. The experiment involved identifying genes expressed differentially in alcohol-induced and non-induced animals but the following list of candidate genes are only for alcohol-induced states.

On mouse Chromosome 11, candidate genes identified for Alcw3 (18 cM) are Hba-a1 (16cM) and Gabrg2 (19 cM).

Mapping and Phenotype information for this QTL, its variants and associated markers

J:40532

Authors localized quantitative trait loci involved with alcohol dependence/genetic predisposition to acute alcohol withdrawal in mice. BXD (C57BL/6J x DBA/2J) strains, B6D2 F2 animals, and HAW (high alcohol withdrawal) LAW (low alcohol withdrawal) animals was used to identify a QTL, Alcw3 (LOD = 4.1). This locus is in proximity to D11Mit174 on mouse Chromosome 11 approximately 16-19 cM from the centromere and accounts for about 3% of the total phenotypic variance in alcohol withdrawal between D2 and B6 strains (12% of the genetic variance).

J:36168

Linkage analysis was performed on 21 BXD (B=C57BL/6J; D=DBA/2J) recombinant inbred (RI) strains at a marker resolution of 1 cM to detect loci associated with alcohol withdrawal severity. Parental strain DBA/2J exhibits greater alcohol withdrawal severity compared to C57BL/6J. Loci contributing to high risk of withdrawal, Alcw1 and Alcw2, were detected at distal mouse Chromosome 1 with a LOD score of 5.8, and at 38 cM on mouse Chromosome 4 with a LOD score of 5.7 near the b locus (Tyrp1), respectively. DBA/2J-derived alleles confer increased alcohol withdrawal severity at Alcw1 and Alcw2. A locus contributing to protection from severe alcohol withdrawal mapped to 20 cM on mouse Chromosome 11 with a LOD score of 4.1 near D11Mit174. DBA/2J-derived alleles confer decreased alcohol withdrawal severity at Alcw3.

References

• Original: J:36168 Crabbe J, Quantitative trait locus gene mapping: a new method for locating alcohol response genes. Addict Biol. 1996;1(3):229-35
• All: 1 reference(s)