Trp53^tm1Ldo
Targeted Allele Detail

Summary

• Symbol: Trp53^tm1Ldo
• Name: transformation related protein 53; targeted mutation 1, Lawrence A Donehower
• MGI ID: MGI:2159430
• Synonyms: p53^m
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm1Ldo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73757
• Parent Cell Line: AB1 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Intragenic deletion
• Mutation details: Insertion of a PGK-hypoxanthine phosphoribosyltransferase (HPRT) expression cassette introduced into exon 7 an A-to-T mutation that results in a p.Arg245Trp substitution, and deleted 20 kb of sequence that included exons 1-6 and a large undefined upstream region. The authors propose that an unknown upstream promoter initiates a chimeric mRNA containing a 55-nucleotide leader spliced onto exons 7-11. This mRNA contains three in-frame AUG codons. The third AUG codon is a viable translation initiation site, which may produce a C-terminal protein fragment. RT-PCR analyses detect expression of this allele in several tissues of heterozygous mutant mice, albeit at lower levels than the wild-type allele. Studies of in vitro transcription and translation of a construct containing cDNA from this allele detected expression of a predicted 24 kDa protein. (J:73757)

Expression

In Structures Affected by this Mutation:

12 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Trp53^tm1Ldo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 240 strains or lines available

References

• Original: J:73757 Tyner SD, et al., p53 mutant mice that display early ageing-associated phenotypes. Nature. 2002 Jan 3;415(6867):45-53
• All: 11 reference(s)