Cpb2^tm1Mng
Targeted Allele Detail

Summary

• Symbol: Cpb2^tm1Mng
• Name: carboxypeptidase B2; targeted mutation 1, Mariko Nagashima
• MGI ID: MGI:2159468
• Synonyms: TAFI^-
• Gene: Cpb2 Location: Chr14:75479727-75520995 bp, + strand Genetic Position: Chr14, 39.73 cM, cytoband D2
• Alliance: Cpb2^tm1Mng page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73713
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced half of exon 8 and all of exon 9, sequences that encode zinc and substrate binding sites. RT-PCR analysis using primers that span the entire coding region detected a truncated transcript in liver from homozygous mutant mice. Western blot analysis using polyclonal antibodies raised against a peptide encoded by exon 5 did not detect protein in plasma from homozygous mutant mice. (J:73713)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cpb2 Mutation: 31 strains or lines available

References

• Original: J:73713 Nagashima M, et al., Thrombin-activatable fibrinolysis inhibitor (TAFI) deficiency is compatible with murine life. J Clin Invest. 2002 Jan;109(1):101-10
• All: 6 reference(s)