Inpp5b^tm2.1Nbm
Targeted Allele Detail

Summary

• Symbol: Inpp5b^tm2.1Nbm
• Name: inositol polyphosphate-5-phosphatase B; targeted mutation 2.1, Robert L Nussbaum
• MGI ID: MGI:2159620
• Synonyms: Inpp5b^tr
• Gene: Inpp5b Location: Chr4:124635643-124695304 bp, + strand Genetic Position: Chr4, 57.89 cM, cytoband D2
• Alliance: Inpp5b^tm2.1Nbm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73678
• Parent Cell Line: TC1/TC-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele is a derivative of Inpp5b^tm2Nbm in which Cre-mediated recombination in vivo under control of an EIIa promoter excised both the neomycin resistance cassette and exon 15 in the germline. Northern blot analysis using a 3' UTR probe did not detect either transcript in testis from homozygous mutant mice. RT-PCR analysis of testis with primers flanking exon 15 detected a deletion of 137 bp in the RNA transcribed by this allele. (J:73678)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Inpp5b Mutation: 57 strains or lines available

References

• Original: J:73678 Hellsten E, et al., Disrupted sperm function and fertilin beta processing in mice deficient in the inositol polyphosphate 5-phosphatase Inpp5b. Dev Biol. 2001 Dec 15;240(2):641-53
• All: 1 reference(s)