Hoxb8^tm1.1Mrc
Targeted Allele Detail

Summary

• Symbol: Hoxb8^tm1.1Mrc
• Name: homeobox B8; targeted mutation 1, Mario R Capecchi
• MGI ID: MGI:2159735
• Synonyms: Hoxb8^lox
• Gene: Hoxb8 Location: Chr11:96172731-96176141 bp, + strand Genetic Position: Chr11, 59.82 cM
• Alliance: Hoxb8^tm1.1Mrc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73745
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: Insertion of a floxed neomycin resistance cassette into the second exon introduced a premature translation stop codon into the open reading frame. Pronuclear injection of pMC1-Cre into fertilized eggs carrying the Hoxb8^tm1Mrc allele removed the neo cassette, leaving a single loxP site in the second exon. RT-PCR studies of brain detected a longer transcript produced by this allele than by the wild-type allele. (J:73745)

Disease models

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxb8 Mutation: 8 strains or lines available

References

• Original: J:73745 Greer JM, et al., Hoxb8 is required for normal grooming behavior in mice. Neuron. 2002 Jan 3;33(1):23-34
• All: 4 reference(s)