Casp8^tm1.1Yuan
Targeted Allele Detail

Summary

• Symbol: Casp8^tm1.1Yuan
• Name: caspase 8; targeted mutation 1.1, Junying Yuan
• MGI ID: MGI:2176089
• Gene: Casp8 Location: Chr1:58834553-58886663 bp, + strand Genetic Position: Chr1, 29.19 cM, cytoband B
• Alliance: Casp8^tm1.1Yuan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:49459
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele is a derivative of Casp8^tm1Yuan in which the loxP-flanked neomycin resistance cassette was removed by Cre-mediated recombination by mating mice carrying this allele to mice expressing Cre in the maternal germline. (J:49459)

Phenotypes

Key:

hm	homozygous	ht	heterozygous	tg	involves transgenes	√	phenotype observed
cn	conditional genotype	cx	complex: > 1 genome feature	ot	other: hemizygous, indeterminate,...	N	normal phenotype

Genotype/
Background:

	Allelic Composition	Genetic Background	Cell Line(s)

	Allelic Composition	Genetic Background	Cell Line(s)
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hm1	Casp8^tm1.1Yuan/Casp8^tm1.1Yuan	involves: 129S1/Sv * 129X1/SvJ * BALB/c * C57BL/6 * MF1
cn2	Casp8^tm1Wll/Casp8^tm1.1Yuan Tg(Mx1-cre)1Cgn/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * CBA * MF1
cn3	Casp8^tm1Wll/Casp8^tm1.1Yuan Tg(Alb1-cre)7Gsc/0	involves: 129S1/Sv * 129X1/SvJ * MF1
cn4	Casp8^tm1Wll/Casp8^tm1.1Yuan Tg(Tie1-cre)9Ref/0	involves: 129S1/Sv * 129X1/SvJ * MF1
cn5	Casp8^tm1Wll/Casp8^tm1.1Yuan Lyz2^tm1(cre)Ifo/Lyz2^+	involves: 129S1/Sv * 129X1/SvJ * MF1

Phenotypes:

Affected Systems	hm1	cn2	cn3	cn4	cn5
show or hide all annotated terms
cardiovascular system	√			√
abnormal vitelline vasculature morphology				√
visceral vascular congestion				√
abnormal myocardium layer morphology	√
myocardium necrosis				√
globular heart				√
thin ventricular wall				√
pericardial edema				√
abnormal blood circulation	√
cellular					√
abnormal macrophage differentiation					√
embryo	√			√
abnormal vitelline vasculature morphology				√
decreased embryo size	√
wavy neural tube				√
abnormal visceral yolk sac morphology				√
growth/size/body	√
decreased embryo size	√
hematopoietic system	√	√		N	√
hematopoietic system phenotype				N
abnormal hematopoietic system morphology/development	√
abnormal myeloblast morphology/development		√
abnormal macrophage differentiation					√
homeostasis/metabolism			√	√
pericardial edema				√
abnormal response/metabolism to endogenous compounds			√
immune system					√
abnormal macrophage differentiation					√
mortality/aging	√	√	√	√
decreased susceptibility to induced morbidity/mortality		√	√
prenatal lethality, complete penetrance	√
embryonic lethality during organogenesis, complete penetrance				√
muscle	√			√
abnormal myocardium layer morphology	√
myocardium necrosis				√
nervous system				√
wavy neural tube				√

View phenotypes and curated references for all genotypes (concatenated display).

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	9 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Casp8 Mutation: 45 strains or lines available

References

• Original: J:49459 Varfolomeev EE, et al., Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas/Apo1, and DR3 and is lethal prenatally. Immunity. 1998 Aug;9(2):267-76
• All: 7 reference(s)