Idua^tm1Clk
Targeted Allele Detail

Summary

• Symbol: Idua^tm1Clk
• Name: iduronidase, alpha-L; targeted mutation 1, Lorne A Clarke
• MGI ID: MGI:2176236
• Gene: Idua Location: Chr5:108808197-108832423 bp, + strand Genetic Position: Chr5, 53.24 cM
• Alliance: Idua^tm1Clk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:39522
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin selection cassette was inserted into exon 6. RT-PCR analysis on RNA derived from liver and kidney of homozygous mice demonstrated that no detectable transcript was produced from this allele. Enzymatic activity assays on homogenates of liver, kidney, brain and tail clippings of homozygous mice confirmed that no functional protein was expressed from this allele. (J:39522)

Disease models

Expression

In Structures Affected by this Mutation:

19 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Idua Mutation: 41 strains or lines available

References

• Original: J:39522 Clarke LA, et al., Murine mucopolysaccharidosis type I: targeted disruption of the murine alpha-L-iduronidase gene. Hum Mol Genet. 1997 Apr;6(4):503-11
• All: 32 reference(s)