Mapk10^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Mapk10^tm1Flv
• Name: mitogen-activated protein kinase 10; targeted mutation 1, Richard A Flavell
• MGI ID: MGI:2176237
• Synonyms: Jnk3-
• Gene: Mapk10 Location: Chr5:103056413-103359200 bp, - strand Genetic Position: Chr5, 49.61 cM, cytoband E4
• Alliance: Mapk10^tm1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:43658
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing sequences corresponding to amino acids 211-267, which encode the tripeptide dual phosphorylation motif required for protein kinase activity. Northern blot and RT-PCR analysis on total brain RNA derived from homozygous mice demonstrated that no detectable transcript is produced from this allele. Enzymatic activity assays on extracts derived from hippocampus of homozygous mice confirmed that no functional protein was expressed from this allele. (J:43658)

Expression

In Mice Carrying this Mutation:	80 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mapk10 Mutation: 37 strains or lines available

References

• Original: J:43658 Yang DD, et al., Absence of excitotoxicity-induced apoptosis in the hippocampus of mice lacking the Jnk3 gene. Nature. 1997 Oct 23;389(6653):865-70
• All: 41 reference(s)