Zap70^tm1Weis
Targeted Allele Detail

Summary

• Symbol: Zap70^tm1Weis
• Name: zeta-chain (TCR) associated protein kinase; targeted mutation 1, Arthur Weiss
• MGI ID: MGI:2176252
• Synonyms: ZAP-70 -
• Gene: Zap70 Location: Chr1:36800912-36821899 bp, + strand Genetic Position: Chr1, 15.41 cM, cytoband A4-C1
• Alliance: Zap70^tm1Weis page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:74974
• Parent Cell Line: JM-1 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing sequences corresponding to amino acids 221 to 520, which encode the C-terminal SH2 domain. Western blot analysis on thymocytes derived from homozygous mice confirmed that no detectable protein is expressed from this allele. (J:74974)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Zap70 Mutation: 55 strains or lines available

References

• Original: J:74974 Kadlecek TA, et al., Differential requirements for ZAP-70 in TCR signaling and T cell development. J Immunol. 1998 Nov 1;161(9):4688-94
• All: 27 reference(s)