Smo^tm2Amc
Targeted Allele Detail

Summary

• Symbol: Smo^tm2Amc
• Name: smoothened, frizzled class receptor; targeted mutation 2, Andrew P McMahon
• MGI ID: MGI:2176256
• Synonyms: Smo^c, Smo^f, Smo^fl, Smo^flox
• Gene: Smo Location: Chr6:29735502-29761364 bp, + strand Genetic Position: Chr6, 12.36 cM, cytoband A3.3
• Alliance: Smo^tm2Amc page

Intestinal mesenchymal proliferation and expansion are affected in Smo^tm2Amc/Smo^tm2Amc Nkx3-2^tm1(cre)Wez/Nkx3-2⁺ embryos

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:73071
• Parent Cell Line: AV3 (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change, Reporter)
• Mutation: Insertion
• Mutation details: Two loxP sites and an frt-flanked neomycin resistance cassette were inserted flanking ~400 bp of the first coding exon 1 from 44 bp upstream through 358 bp downstream of the translation initiation codon. A tau-LacZ gene was also inserted 3' to the neomycin resistance gene. (J:73071)

Expression

In Mice Carrying this Mutation:	356 assay results 4 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	65 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Smo^tm2Amc

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Smo Mutation: 39 strains or lines available

References

• Original: J:73071 Long F, et al., Genetic manipulation of hedgehog signaling in the endochondral skeleton reveals a direct role in the regulation of chondrocyte proliferation. Development. 2001 Dec;128(24):5099-108
• All: 203 reference(s)