Marcks^tm1Pjb
Targeted Allele Detail

Summary

• Symbol: Marcks^tm1Pjb
• Name: myristoylated alanine rich protein kinase C substrate; targeted mutation 1, Perry J Blackshear
• MGI ID: MGI:2176302
• Synonyms: MARCKS^-
• Gene: Marcks Location: Chr10:37009371-37014916 bp, - strand Genetic Position: Chr10, 19.49 cM
• Alliance: Marcks^tm1Pjb page

Coronal sections of brains from Marcks^tm1Pjb/Marcks^tm1Pjb mice at E18.5

Show the 3 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:22949
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A neomycin resistance gene was inserted into exon 1, 18 bp after the ATG initiation codon. Northern blot analysis on RNA derived from E18.5 embryonic heads of homozygous mice demonstrated that no detectable transcript was produced from this allele. Western blot analysis on on lysates also of E18.5 embryonic heads of homozygous mice confirmed that no detectable protein was expressed from this allele. (J:22949)

Expression

In Mice Carrying this Mutation:	17 assay results
In Structures Affected by this Mutation:	13 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Marcks Mutation: 13 strains or lines available

References

• Original: J:22949 Stumpo DJ, et al., MARCKS deficiency in mice leads to abnormal brain development and perinatal death. Proc Natl Acad Sci U S A. 1995 Feb 14;92(4):944-8
• All: 15 reference(s)