Mif^tm1Dvd
Targeted Allele Detail

Summary

• Symbol: Mif^tm1Dvd
• Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor); targeted mutation 1, John R David
• MGI ID: MGI:2176337
• Synonyms: MIF-, MIF-KO
• Gene: Mif Location: Chr10:75695187-75696074 bp, - strand Genetic Position: Chr10, 38.59 cM, cytoband C1
• Alliance: Mif^tm1Dvd page

BBN-induced bladder cancer is less aggressive in Mif^tm1Dvd/Mif^tm1Dvd mice than in wild type and does not invade muscle layers

Show the 2 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:52300
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin resistance cassette replaced a genomic fragment containing part of exon 2 and exon 3. Northern blot analysis on RNA derived from liver of LPS-treated homozygous mice demonstrated that no detectable transcript was produced form this allele. ELISA assays on serum of LPS-treated animals confirmed that no functional protein was expressed from this allele. (J:52300)

Expression

In Mice Carrying this Mutation:	1 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Mif Mutation: 22 strains or lines available

References

• Original: J:52300 Bozza M, et al., Targeted disruption of migration inhibitory factor gene reveals its critical role in sepsis. J Exp Med. 1999 Jan 18;189(2):341-6
• All: 61 reference(s)