Runx2^tm1Kish
Targeted Allele Detail

Summary

• Symbol: Runx2^tm1Kish
• Name: runt related transcription factor 2; targeted mutation 1, Tadamitsu Kishimoto
• MGI ID: MGI:2176466
• Synonyms: Cbfa1-, Runx2^-, Runx2-deltaC, Runx2^tm1Tki
• Gene: Runx2 Location: Chr17:44806873-45125518 bp, - strand Genetic Position: Chr17, 21.33 cM
• Alliance: Runx2^tm1Kish page

Calvaria and clavicle abnormalities in Gsk3b^tm1Jrw/Gsk3b⁺, Runx2^tm1Kish/Runx2⁺, and Gsk3b^tm1Jrw/Gsk3b⁺ Runx2^tm1Kish/Runx2⁺ mice

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:40783
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A PGK-neomycin resistance cassette replaced 1.2 kb of exon 1, which encodes the first 41 amino acids of the runt domain. RT-PCR analysis of the runt region did not detect cDNA in liver from homozygous mice. (J:40783)

Disease models

Expression

In Mice Carrying this Mutation:	126 assay results
In Structures Affected by this Mutation:	23 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Runx2 Mutation: 44 strains or lines available

References

• Original: J:40783 Komori T, et al., Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell. 1997 May 30;89(5):755-64
• All: 39 reference(s)